Data Acquisition

Data Analysis

Experimental Design

Training

Cell Sorting

The Flow Cytometry Unit is located in the Core Research Facility, Room H1710 in the Health Sciences Centre. We house two Beckman Coulter CytoFLEX Flow Cytometers, a Beckman Coulter CytoFLEX SRT Cell Sorter, and a Beckman Coulter MoFlo Astrios EQ Cell Sorter. The unit operates on a fee-for-service basis and is available to both internal and external users.

Hours of Operation

Monday through Friday
9:00am - 5:00pm (Fall & Winter)
9:00am - 4:30pm (Summer)

Equipment and Services

Beckman Coulter CytoFLEX Flow Cytometer Tube Reader 3 lasers - 405/488/638nm with 10 parameters (see link) Data acquisition and analysis Programs for analysis include: Kaluza, CyteExpert and FloJo User training is provided for a fee
Beckman Coulter CytoFLEX Flow Cytometer Plate Reader 3 lasers- 405/488/638 with 10 parameters (see link)  Accommodates standard 96-well plates  Data acquisition and analysis  Programs for analysis include: Kaluza, CyteExpert, and FlowJo  User training is provided for a fee
Beckman Coulter CytoFLEX SRT Cell Sorter 4 Lasers-405/488/561/640 with 15 parameters (see link) Data acquisition and analysis 4-way cell sorting All samples must be filtered with a 40 micron nylon mesh cell strainer prior to sorting (BD Falcon, P/N 352235) A live/dead marker is strongly recommended for cell sorting Programs for analysis include: Summit, Kaluza, and FlowJo

Beckman Coulter MoFlo Astrios EQ-Cell Sorter 3 Lasers-405/488/640 nm with 11 parameters (see link) Data acquisition and analysis Cell sorting up to six-ways. Collection into microtitre plates, slides, centrifuge tubes All samples must be filtered with a 40 micron nylon mesh cell strainer prior to sorting (BD Falcon, P/N 352235) A live/dead marker is strongly recommended for cell sorting Programs for analysis include: Summit, Kaluza, and FloJo

Scheduling and Data Policies

• Flow cytometer bookings can be made using an online booking system. Booking system access is provided once a user has completed training.
• Inquiries for cell sorting experiments can be directed to staff in person (room H1710) or via email (see below)
• Cell sorting experiments require a minimum of 24 h notice with staff approval prior to runs
• Cell sorting is staff operated only
• A requisition is required at time of use
• Users are responsible for removing data files at time of acquisition

Biosafety Guidelines

• For all biosafety guidelines refer to Memorial University Biosafety Policies and Procedures Manual.
• All samples transported to the laboratory must be in a sealed container according to biosafety
• Laboratory coats and PPE must be worn at all times. 
• Please Note: Users must add room H1710 to their current Biosafety certificate to use the facility.

Acknowledgements

Please remember to acknowledge any contributions of the Core Research Facility in your publications, presentations, posters, and grant applications.

If the staff have provided technical and scientific advice beyond the routine, please consider them for authorship in your publications if appropriate.  Otherwise, a simple recognition in the acknowledgments sections such as the following is appreciated: “We thank Memorial University’s Core Research Facility, Medical Laboratories for their support with this work.”

Educational Resources
 
Beckman Coulter
Becton Dickinson
ThermoFisher

Spectra of Fluorochromes

FluoroFinder
BioLegend
ThermoFisher

Mailing Address:

Core Research Facility
Health Sciences Centre, Room H1710
Faculty of Medicine
Memorial University of Newfoundland
300 Prince Philip Drive
St. John’s, NL, A1B 3V6
Canada

Contact Persons: