Functional Genomics and Molecular Biology
ARC utilizes functional genomics and molecular biology tools and techniques to support aquatic-based research conducted in (or in collaboration with) the Department of Ocean Sciences (DOS), Memorial University of Newfoundland. Other users will be considered upon request.
Primarily fish and invertebrate samples are submitted to ARC for preparation (e.g. RNA and DNA isolation, cDNA synthesis) and then subjected to downstream analyses [e.g. real-time quantitative polymerase chain reaction (qPCR) analysis, suppression subtractive hybridization (SSH) cDNA library construction, microarray analysis, DNA subcloning]. Assays for qPCR can be custom-designed and are subjected to quality assurance testing. Data analysis is conducted by ARC and can include graphing and statistical analysis. Data, methods and results can be prepared for publication.
Training can be provided to undergraduate honours students, graduate students, post-doctoral fellows and staff on all of the aforementioned tools, techniques and data analysis methods.
ARC instrumentation includes the Applied Biosystems® QuantStudio™ 6 Flex Real-Time PCR System. All reagents and additional equipment must be supplied by the client.