Experiments currently available on Bruker Avance 500
Obs. nuclei |
Exp. Name |
Parameters |
Description |
1H |
1d_1H_12ppm pulse prog.: zg30 |
1H observe with 30o pulse SW: 12 ppm, TD: 32k, O1p: 5 ppm, D1: 3 s, ns: 16. exp. time: 2 mins |
Provides chemical shift and JH-X couplings information for structure determination. Adjustable parameters: NS (number of scans) |
1d_1H_20ppm pulse prog.: zg30 |
1H observe with 30o pulse SW: 20 ppm, TD: 65k, O1p: 5 ppm, d1: 3 s, ns: 16. exp. time: 2mins |
Used to minimize/eliminate the intense water signal for samples in H2O/D2O solvent. Adjustable parameters: TD0 (number of cycle) |
|
1d_1H_PRESAT pulse prog.: zgpr |
1H observe with water suppression SW: 12 ppm, TD: 65k, O1p: 4.7ppm, d1: 2s, ns: 16 exp. time: 2 mins |
Used to minimize/eliminate the intense water signal for samples in H2O/D2O solvent. Adjustable parameters: NS (number of scans) |
|
1d_DOSY pulse prog:stegp1s1d |
1H observe diffusion measurement using stimulated echo SW: 12 ppm, TD: 16k, O1p: 5 ppm, D1: 3 s, ns: 8. exp. time: 1 mins |
1D diffusion experiment used for set-up of 2d_DOSY. Adjustable parameters: D20 (big delta), p30 (little delta), GPZ6 (gradient strength % - start at 2, compare with 95) |
|
1d_HOMODEC pulse prog.: zg0hd |
1H observe with homodecoupling TD1: 32k, sw: 12 ppm, O1p: 5 ppm, d1: 3 s, ns: 16 expt: 2 mins |
Used for decoupling protons from protons. You will need to first run a regular 1H spectrum to measure position of the signal to irradiate. Adjustable parameter: O2p (position of the irradiated proton). |
|
1d_MULTIPRESAT pulse prog.: lc1pnps |
1H observe with multiple solvent suppression TD1: 32k, sw: 20 ppm, O1p: 5 ppm, d1: 3 s, ns: 16 expt: 10 mins. |
Use shape pulse for off-resonance presaturation of multiple peaks. Use “xau lcsetup” in command line to setup experiment. Use “xau au_lc1d” in command line to start experiment. |
|
1H |
2d_COSY pulse prog.: cosygpqf |
2D homonuclear shift correlation using gradient pulse for selection TD2: 2048, TD1: 256, d1: 1.5 s, ns: 1 expt: 8 mins |
Used for correlating protons over 2- or more bonds. Adjustable parameters: NS (number of scans) |
2d_DOSY pulse prog.: stegp1s |
2D diffusion experiment SW: 12 ppm, TD: 16k, O1p: 5 ppm, d1: 3s, ns: 8 expt: variable |
Used to measure diffusion coefficient or to separate compounds of different size. Must use 1d_DOSY to setup experiment (optimize big delta, small delta). Experiment starts with DOSY in command line. Adjustable parameters: D20 (big delta), p30 (little delta), NS (number of scans), RG (gain) |
|
2d_HSQC pulse prog.: hsqcetgpsi |
2D 1H-13C correlation via double inept transfer using trim pulses in inept transfer, decoupling during acquisition, phase sensitive using Echo/Antiecho-TPPI gradient selection. TD2: 2k, TD1: 256, d1: 1.5, ns:2, sw2: 20ppm, sw1: 300ppm. expt: 14 min |
Correlates 1H and 13C connected by C-H coupling over 1 bond. Adjustable parameters: NS (number of scans) |
|
2d_NOESY pulse prog.: noesygpph |
2D homonuclear correlation via dipolar coupling, using gradient pulse in mixing time, phase sensitive (States-TPPI) TD2: 2K, TD1: 256, d1: 1.5s, d8: 800 ms , ns: 2, sw: 12ppm expt: 21 mins. |
Establishing NOEs. Dipolar coupling may be due to NOE or chemical exchange. Suitable for small (Mr<1000) and large (Mr>2000) molecules (positive and negative NOE, respectively). Adjustable parameters: NS (number of scans), d8: mixing time (value about T1) |
|
2d_T1_inv_reco pulse prog.:t1ir |
1H observe T1 measurement using inversion recovery SW: 12ppm TD: 16k, O1p: 5 ppm, d1: 10s, ns: 8 expt: 22 min |
Pseudo-2D used to measure T1. Adjustable parameters: NS (number of scans), VDLIST (variable delay list) |
|
2d_T2 pulse prog.: cpmg_vd |
1H observe T2 measurement using Carr-Purcell_meiboom-Gill sequence . SW: 12ppm TD: 16k, O1p: 5 ppm, d1: 10s, ns: 8 expt: 20 min |
Pseudo-2D used to measure T1. Adjustable parameters: NS (number of scans), VDLIST (variable delay list) |