Experiments currently available on Bruker Avance 300 | CREAIT Network

This list is not exhaustive, experiments will be added on request.

Obs. nuclei	Exp. Name	Parameters	Description
¹H	N_PROTON pulse prog.: zg30	¹H observe with 30^o pulse SW: 20.6 ppm, TD: 65k, O1p: 6.175 ppm, D1: 1 s, ns: 16. exp. time: 2 mins	Provides chemical shift and J_H-Xcouplings information for structure determination. Adjustable parameters: TD0 (number of cycle)
	N_WATERSUP pulse prog.: zgpr	¹H observe with water supression SW: 12.12 ppm, TD: 32k, O1p: 4.7 ppm, d1: 5 s, ns: 8. exp. time: 2mins	Used to minimize/eliminate the intense water signal for samples in H₂O/D₂O solvent. Adjustable parameters: TD0 (number of cycle)
	N_H1para pulse prog.: zg30	¹H observe with 30^o pulse, extra wide sweep width SW: 50 ppm, TD: 65k, O1p: 0 ppm, d1: 1s, ns: 16 exp. time: 2 mins	Used on paramagnetic samples. Provides chemical shift and JH-X couplings information for structure determination Adjustable parameters: TD0 (number of cycle)
	N_1H_VT pulse prog: zg30	¹H observe with 30^o pulse, for vaiable temperature SW: 20.6 ppm, TD: 65k, O1p: 6.175 ppm, D1: 1 s, ns: 16. exp. time: 2 mins	Variable temperature experiment. Provides chemical shift and JH-X couplings information for structure determination. Adjustable parameters: TD0 (number of cycle), TE (temperature)
	C_COSYGPSW pulse prog.: cosygpqf	2D homonuclear shift correlation using gradient pulse for selection TD2: 2048, TD1: 128, d1: 1.5 s, ns: 1 expt: 4 mins	Used for correlating protons over 2- or more bonds. This is a composite experiment. It will first run a ¹H spectrum to adjust SW and O1p. Magnitude mode phasing.
	C_NOESYGPPH pulse prog.: noesygpph	2D homonuclear correlation via dipolar coupling, using gradient pulse in mixing time, phase sensitive (States-TPPI) TD2: 1K, TD1: 256, d1: 1.3 s, d8 (mixing time): 500 ms , ns: 4 expt: 43 mins.	Establishing NOEs. Dipolar coupling may be due to NOE or chemical exchange. Suitable for small (Mr<1000) and large (Mr>2000) molecules (positive and negative NOE, respectively). This is a composite experiment. It will first run a 1H spectrum to adjust SW and O1p.
¹³C	N_C13CPD_256 pulse prog.: zgpg30	¹³C observe with ¹H power-gated decoupling, using 30^o pulse SW: 238.8 ppm, TD: 65k, O1p: 100 ppm, d1: 2s, ns: 256 expt: 17 mins	Provides chemical shift information for structure determination. NS optimized for 20mg/ml concentration. Adjustable parameters: TD0 (number of cycle)
	N_C13CPD_overnight pulse prog.: zgpg30	¹³C observe with ¹H power-gated decoupling, using 30^o pulse SW: 238.8 ppm, TD: 65k, O1p: 100 ppm, d1: 2s, ns: 256 expt: 8h	Overnight experiment. Provides chemical shift information for structure determination. NS optimized for low concentration <5mg/ml.
	N_C13DEPT135_16 pulse prog.: dept135	¹³C observe with Distortionless Enhancement of the ¹³C signal strength by Polarization Transfer from proton to carbon using 135° proton pulse SW: 238.8 ppm, TD: 65k, O1p: 100ppm, d1: 2 s, ns: 16 expt: 1 min	Distinguishes between CH₃/CH (normally shown in a positive phase) and CH₂ (negative phase); quaternary carbons are not detected. NS optimized for 20mg/ml concentration Adjustable parameters: TD0 (number of cycle)
	N_C13APT_16 pulse prog.:jmod	¹³C observe with J-modulated spin-echo coupled to proton to determine a number of attached protons SW: 238.8 ppm, TD: 65k, O1p: 100 ppm, d1: 2s, ns: 16 expt: 4 min	Distinguishes between CH₃/CH (normally shown in a positive phase) and CH₂/C (negative phase). NS optimized for 20mg/ml concentration Adjustable parameters: TD0 (number of cycle)
	N_C13GD pulse prog.:zggd30	¹³C observe with gated decoupling using 30 degree flip angle SW: 238.8 ppm TD: 65k, O1p: 100 ppm, d1: 2s, ns: 1k expt: 2 min	Observe ¹H-coupled spectrum. Provides J_C-Hcouplings information. NS optimized for 20mg/ml concentration Adjustable parameters: TD0 (number of cycle)
	C_HSQCEDETGT pulse prog.: hsqcedetgp	2D ¹H-¹³C correlation via double inept transfer with multiplicity editing during selection step, using trim pulses in inept transfer, decoupling during acquisition, phase sensitive using Echo/Antiecho-TPPI gradient selection. TD2: 1k, TD1: 256, d1: 1.5, ns:2 expt: 14 min	Correlates ¹H and ¹³C connected by C-H coupling over 1 bond. Destinguishes between CH₃, CH (generally positive) and CH₂ (negative). This is a composite experiment. It will first run a ¹H spectrum to adjust SW and O1p.
¹⁹F	N_F19CPD pulse prog.: zgfhigqn	¹⁹F observe with inverse gated ¹H decoupling SW: 237 ppm, TD: 130k, d1: 1 s, ns: 16. Referenced to CFCl₃ at 0 ppm. expt: 40s	Provides chemical shift information for structure determination and J_F-X couplings information. Adjustable parameters: TD0 (number of cycle)
³¹P	N_P31CPD pulse prog.: zgpg30	³¹P observe with inverse gated ¹H decoupling SW: 403 ppm, TD: 65k, d1: 2 s, ns: 16. Referenced to H₃PO₄ at 0 ppm. expt: 1 min	Provides chemical shift information for structure determination and J_P-Xcouplings information. Adjustable parameters: TD0 (number of cycle)
²⁹Si	N_Si29IG pulse prog.: zgig	²⁹Si observe with inverse gated decoupling SW: 400 ppm, TD: 65k, d1: 5s, ns: 128. Referenced to 1% TMS at 0 ppm. expt: 14min	Provides chemical shift information for structure determination and J_Si-X couplings information Require special silicon-free tube. Adjustable parameters: TD0 (number of cycle)
	N_Si29INEPT pulse prog.: ineptrd	²⁹Si observe with INEPT for non-selective polarization transfer with decoupling during acquisition SW: 400 ppm, TD: 32k, d1: 5s, ns: 256. Referenced to 1% TMS at 0 ppm. expt: 24 min	Provides chemical shift information for structure determination and J_Si-X couplings information. Only works for protonated Si. Adjustable parameters: TD0 (number of cycle)
²⁷Al	N_Al27_16 pulse prog.: zg	²⁷Al observe SW: 500 ppm, TD: 32k, d1: 1s, ns: 16. Referenced to Al(NO₃)₃ at 0 ppm. expt: 25s	Provides chemical shift information for structure determination Adjustable parameters: TD0 (number of cycle)
¹⁵N	N_N15INEPT pulse prog.: ineptrd	¹⁵N observe with INEPT for non-selective polarization transfer with decoupling during acquisition SW: 500 ppm, TD: 32k, d1: 5s, ns: 32. expt: 4 min	Provides chemical shift information for structure determination Requires ¹⁵N-enriched sample Adjustable parameters: TD0 (number of cycle)
⁷Li	N-Li7zg30 pulse prog.: zg30	⁷Li observe with 30 degree pulse SW: 200 ppm, TD: 16k, d1: 5s, ns: 16. Referenced to 20mg/ml LiCl at 0 ppm. expt: 2 min	Provides chemical shift information for structure determination Adjustable parameters: TD0 (number of cycle)
¹¹B	N_B11_16 pulse prog.: zg	¹¹B observe SW: 200 ppm, TD: 64k, d1: 1s, ns: 16. Referenced to 5% BF₃.OEt₃ at 0 ppm. expt: 1 min	Provides chemical shift information for structure determination Adjustable parameters: TD0 (number of cycle)

Obs. nuclei

Exp. Name

Parameters

Description

¹H

N_PROTON

pulse prog.: zg30

¹H observe with 30^o pulse

SW: 20.6 ppm, TD: 65k, O1p: 6.175 ppm, D1: 1 s, ns: 16.

exp. time: 2 mins

Provides chemical shift and J_H-Xcouplings information for structure determination.

Adjustable parameters: TD0 (number of cycle)

N_WATERSUP

pulse prog.: zgpr

¹H observe with water supression

SW: 12.12 ppm, TD: 32k, O1p: 4.7 ppm, d1: 5 s, ns: 8.

exp. time: 2mins

Used to minimize/eliminate the intense water signal for samples in H₂O/D₂O solvent.

Adjustable parameters: TD0 (number of cycle)

N_H1para

pulse prog.: zg30

¹H observe with 30^o pulse, extra wide sweep width

SW: 50 ppm, TD: 65k, O1p: 0 ppm, d1: 1s, ns: 16

exp. time: 2 mins

Used on paramagnetic samples. Provides chemical shift and JH-X couplings information for structure determination

Adjustable parameters: TD0 (number of cycle)

N_1H_VT

pulse prog: zg30

¹H observe with 30^o pulse, for vaiable temperature

SW: 20.6 ppm, TD: 65k, O1p: 6.175 ppm, D1: 1 s, ns: 16.

exp. time: 2 mins

Variable temperature experiment. Provides chemical shift and JH-X couplings information for structure determination.

Adjustable parameters: TD0 (number of cycle), TE (temperature)

C_COSYGPSW

pulse prog.: cosygpqf

2D homonuclear shift correlation using gradient pulse for selection

TD2: 2048, TD1: 128, d1: 1.5 s, ns: 1

expt: 4 mins

Used for correlating protons over 2- or more bonds.

This is a composite experiment. It will first run a ¹H spectrum to adjust SW and O1p.

Magnitude mode phasing.

C_NOESYGPPH

pulse prog.: noesygpph

2D homonuclear correlation via dipolar coupling, using gradient pulse in mixing time, phase sensitive (States-TPPI)

TD2: 1K, TD1: 256, d1: 1.3 s, d8 (mixing time): 500 ms , ns: 4

expt: 43 mins.

Establishing NOEs. Dipolar coupling may be due to NOE or chemical exchange. Suitable for small (Mr<1000) and large (Mr>2000) molecules (positive and negative NOE, respectively).

This is a composite experiment. It will first run a 1H spectrum to adjust SW and O1p.

¹³C

N_C13CPD_256

pulse prog.: zgpg30

¹³C observe with ¹H power-gated decoupling, using 30^o pulse

SW: 238.8 ppm, TD: 65k, O1p: 100 ppm, d1: 2s, ns: 256

expt: 17 mins

Provides chemical shift information for structure determination.

NS optimized for 20mg/ml concentration.

Adjustable parameters: TD0 (number of cycle)

N_C13CPD_overnight

pulse prog.: zgpg30

¹³C observe with ¹H power-gated decoupling, using 30^o pulse

SW: 238.8 ppm, TD: 65k, O1p: 100 ppm, d1: 2s, ns: 256

expt: 8h

Overnight experiment. Provides chemical shift information for structure determination.

NS optimized for low concentration <5mg/ml.

N_C13DEPT135_16

pulse prog.: dept135

¹³C observe with Distortionless Enhancement of the ¹³C signal strength by Polarization Transfer from proton to carbon using 135° proton pulse

SW: 238.8 ppm, TD: 65k, O1p: 100ppm, d1: 2 s, ns: 16

expt: 1 min

Distinguishes between CH₃/CH (normally shown in a positive phase) and CH₂ (negative phase); quaternary carbons are not detected.

NS optimized for 20mg/ml concentration

Adjustable parameters: TD0 (number of cycle)

N_C13APT_16

pulse prog.:jmod

¹³C observe with J-modulated spin-echo coupled to proton to determine a number of attached protons

SW: 238.8 ppm, TD: 65k, O1p: 100 ppm, d1: 2s, ns: 16

expt: 4 min

Distinguishes between CH₃/CH (normally shown in a positive phase) and CH₂/C (negative phase).

NS optimized for 20mg/ml concentration

Adjustable parameters: TD0 (number of cycle)

N_C13GD

pulse prog.:zggd30

¹³C observe with gated decoupling using 30 degree flip angle

SW: 238.8 ppm TD: 65k, O1p: 100 ppm, d1: 2s, ns: 1k

expt: 2 min

Observe ¹H-coupled spectrum. Provides J_C-Hcouplings information.

NS optimized for 20mg/ml concentration

Adjustable parameters: TD0 (number of cycle)

C_HSQCEDETGT

pulse prog.: hsqcedetgp

2D ¹H-¹³C correlation via double inept transfer with multiplicity editing during selection step, using trim pulses in inept transfer, decoupling during acquisition, phase sensitive using Echo/Antiecho-TPPI gradient selection.

TD2: 1k, TD1: 256, d1: 1.5, ns:2

expt: 14 min

Correlates ¹H and ¹³C connected by C-H coupling over 1 bond. Destinguishes between CH₃, CH (generally positive) and CH₂ (negative).

This is a composite experiment. It will first run a ¹H spectrum to adjust SW and O1p.

¹⁹F

N_F19CPD

pulse prog.: zgfhigqn

¹⁹F observe with inverse gated ¹H decoupling

SW: 237 ppm, TD: 130k, d1: 1 s, ns: 16. Referenced to CFCl₃ at 0 ppm.

expt: 40s

Provides chemical shift information for structure determination and J_F-X couplings information.

Adjustable parameters: TD0 (number of cycle)

³¹P

N_P31CPD

pulse prog.: zgpg30

³¹P observe with inverse gated ¹H decoupling

SW: 403 ppm, TD: 65k, d1: 2 s, ns: 16. Referenced to H₃PO₄ at 0 ppm.

expt: 1 min

Provides chemical shift information for structure determination and J_P-Xcouplings information.

Adjustable parameters: TD0 (number of cycle)

²⁹Si

N_Si29IG

pulse prog.: zgig

²⁹Si observe with inverse gated decoupling

SW: 400 ppm, TD: 65k, d1: 5s, ns: 128. Referenced to 1% TMS at 0 ppm.

expt: 14min

Provides chemical shift information for structure determination and J_Si-X couplings information

Require special silicon-free tube.

Adjustable parameters: TD0 (number of cycle)

N_Si29INEPT

pulse prog.: ineptrd

²⁹Si observe with INEPT for non-selective polarization transfer with decoupling during acquisition

SW: 400 ppm, TD: 32k, d1: 5s, ns: 256. Referenced to 1% TMS at 0 ppm.

expt: 24 min

Provides chemical shift information for structure determination and J_Si-X couplings information.

Only works for protonated Si.

Adjustable parameters: TD0 (number of cycle)

²⁷Al

N_Al27_16

pulse prog.: zg

²⁷Al observe

SW: 500 ppm, TD: 32k, d1: 1s, ns: 16. Referenced to Al(NO₃)₃ at 0 ppm.

expt: 25s

Provides chemical shift information for structure determination

Adjustable parameters: TD0 (number of cycle)

¹⁵N

N_N15INEPT

pulse prog.: ineptrd

¹⁵N observe with INEPT for non-selective polarization transfer with decoupling during acquisition

SW: 500 ppm, TD: 32k, d1: 5s, ns: 32.

expt: 4 min

Provides chemical shift information for structure determination

Requires ¹⁵N-enriched sample

Adjustable parameters: TD0 (number of cycle)

⁷Li

N-Li7zg30

pulse prog.: zg30

⁷Li observe with 30 degree pulse

SW: 200 ppm, TD: 16k, d1: 5s, ns: 16. Referenced to 20mg/ml LiCl at 0 ppm.

expt: 2 min

Provides chemical shift information for structure determination

Adjustable parameters: TD0 (number of cycle)

¹¹B

N_B11_16

pulse prog.: zg

¹¹B observe

SW: 200 ppm, TD: 64k, d1: 1s, ns: 16. Referenced to 5% BF₃.OEt₃ at 0 ppm.

expt: 1 min

Provides chemical shift information for structure determination

Adjustable parameters: TD0 (number of cycle)