Bio3950 - Fundamentals of Genetic Biotechnology

Genomic Biotechnologies for Biodiversity:
        Genomes, Microarrays, & DNA Chips

Genomics: the study of complete gene sets in organisms
     "Genomic Thinking  ": high-volume, high-throughput, massively-parallel experimental approaches
      Biotechnology: The use of biological systems to provide goods & services

      Biodiversity: intra- & inter-specific variation in DNA genomes
                               genomic approaches to conservation & population biology, fisheries & aquaculture

                               Ex.: Genomic variation in Newfoundlanders [PPT presentation]
                               Ex.: Genomic phylogeny
of Codfish & Pollocks
                                                         Phylogeography of Atlantic Cod [PPT presentation]
       Bioinformatics: computer-based extraction & synthesis of DNA information
                               "BLASTing a Sea Monster"

Biotechnology for Biodiversity

Review of Biotechnology (from Bio2250 - Principles of Genetics)
        DNA "Xeroxing" : the Polymerase Chain Reaction (PCR)
        Reading the A C G T code:  Automated DNA sequencing
        Identifying variation in DNA sequences:  Single-Nucleotide Polymorphisms (SNPs)
        Fast detection methods: Southern Blots Allele-Specific Oligonucleotides (ASOs)
                                                                                Species-Specific Oligonucleotides (SSOs)

Biotech 101 - "DNA Chips"
    DNA Re-Sequencing Chips for iterative sequencing of populations [PPT presentation]
The ArkChip for multiplex species populations studies

Biotech 202 - Quantitative "Real Time" PCR
    Species Identification by means of PCR-based ASO tests
    "One fish, Two fish": differential larval survival by means of  rtPCR-ASO tests
    Relative mating success at diploid loci with multiplex tests

Biotech 303 - Microarray measurement of quantitative gene expression
    "Red fish, Blue fish": Differential genome expression in fast & slow-growing fish

Biotech 404 - MALDI-TOF MS
for DNA re-sequencing

All material ©2011 by Steven M. Carr