Bio4241 - Advanced Topics in Genetics

Bio4241 - Advanced Topics in Genetic Analysis

E-mail: scarr@mun.ca
Notes for Bio2250 (Principles of Genetics)
Research in my lab

This course is an extension of Bio2250 to advanced topics in modern Genetics & Genomics.

Bio4241, Winter 2012 semester
        Course Syllabus
        Course Schedule & Outline (Lectures TuTh 1030-1145, En-1052
                                                         Lab           Wed 1400-1700, Sn-4117
        Presentation Schedule & Class List

Final Set, Midterm Exam Questions: 29 Feb 2012 [answer 2 of 4]
Final Exam Questions: 05 April 2012 [answer 3 of 5 questions]

http://www.biol4241.com : an editable domain for student presentations

Introduction to Advanced Topics in Genetics

05 Jan: Orientation & Organization:
(SMC) [10 Jan] Luria & Delbruck (1943). Mutations of bacteria from virus sensitivity to virus resistance. Genetics 28:491. [PDF of original] [PDF of presentation] Posted 03 Jan 2012

[Salvador Luria & Max Delbruck]

[11 &18 Jan Labs] Sn-4117 - Webpage construction & posting to the server
[12 & 17 Jan] Advanced Topics in Mutagenesis

Radiation Genetics: High-Energy mutagenesis
Ames Test: Environmental mutagenesis

Suppressor mutations: "Two wrong make a right"

[19 Jan] The Genetic Code & its role in protein synthesis [see "Cracking the Code"]

Student Presentations
    See the Nobel e-Museum for biographies, summaries & other resources
    See PubMed at NCBI for papers
    Historical material & animations at Cold Spring Harbor Labs DNA Learning Center

(1) [24 Jan] Genetic function of DNA: "³²P goes in, ³⁵S stays out."

Hershey & Chase (1952). Independent functions of viral protein and nucleic acid in growth of bacteriophage. J Gen Physiol 36:39-56 [PDF of original]

[Alfred Hershey & Martha Chase]`[PDF of presentation] Posted 22 Jan

see also: Hershey & Chase (1952). Genetic recombination and heterozygosis in bacteriophage. Cold Spring Harbor Symp Quant Biol 16: 471-479.

(2) [26 Jan] Semi-Conservative Replication of DNA

Meselson & Stahl (1958). The replication of DNA in Escherichia coli. Proc Natl Acad Sci USA 44:671-682. [PDF of original] [PDF of presentation] Posted 25 Jan

[Matthew Meselson & Franklin Stahl] [Technical note on analytical centrifugation]

(3) [31 Jan] Intron / Exon structure: "Split Genes"

Garapin, Cami, Roskam, Kourilsky, Le Pennec, Perrin, Gerlinger, Cochet, & Chambon (1978). Electron microscopy and restriction enzyme mapping reveal additional intervening sequences in the chicken ovalbumin split gene. Cell 14:629-639 [PDF of original] [PDF of presentation] Posted 31 Jan

(4) [02 Feb] Transposable elements: "Jumping Genes"

McClintock (1953). Induction of instability at selected loci in maize. Genetics 38:579-599. [PDF of original] [PDF of presentation] Posted 01 Feb

Transposable elements: "Jumping Genes"

[Barbara McClintock]
see also: Evelyn Fox Keller (1983) A Feeling for the Organism: the Life and Work of Barbara McClintock. WH Freeman..

(5) [07 Feb] Molecular Evolution: "Apes & Humans are 99% similar"

King & Wilson (1975). Evolution at two levels in humans and chimpanzees. Science 188:107-16. [PDF of original] [PDF of presentation] Posted 05 Feb

[Allan C Wilson] [Allan Wilson: Innovator of Science] [use WinDVD player]

(6) [09 Feb] Dideoxy-chain-termination DNA sequencing: "ACGT etc ..."

Sanger, Nicklen, & Coulson (1977). DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463-5467. [PDF of original] [PDF of presentation] Posted 07 Feb

[Fred Sanger]

(8) [14 Feb] DNA Fingerprinting

Jeffreys, Wilson, & Thein (1985). Individual-specific 'fingerprints' of human DNA. Nature 316:76-79. [PDF of original] [PDF of presentation] Posted

(7) [16 Feb] IonTorrent Personal Genome Machine: Next Next Generation DNA sequencing

Rothberg et al. (2011) An integrated semiconductor device enabling non-optical genome sequencing. Nature 475: 348–352 [PDF of original] [PDF of presentation] Posted12 Feb

Winter Break, 20 - 22 Feb: no classes

Part II: AW Griffiths et al., 2002. Modern Genetic Analysis, 2nd ed. Presentation Schedule

Chapter 07 [23 Feb] Bacteriophage & Bacterial Genetics [PDF of presentation] [Group 1]
Chapter 12 [28 Feb] Mutational Dissection [PDF of presentation] [Group 2]
29 Feb MIDTERM EXAM [2-3P, Lab Period]
Chapter 13 [01 Mar] Gene Regulation [PDF of presentation] [Group 3]
Chapter 15 [06 Mar] Regulation of Cell Number: Cancer [PDF of presentation] [Group 4]
Chapter 16 Genetics of Complex phenotypes:

[08 Mar]* Binary Fate & Sex Determination [PDF of presentation] [Group 6]
[13 Mar]* Complex Pattern Formation [PDF of presentation] [Group 5]

Guest [15 Mar] Guest Lecture: Dr Ann Dorward: From gene discovery to gene-environment interactions:

the mouse as a tool for reproductive physiology research [PDF notes]

Chapter TBA Post-Modern Genetics

SMC [20 Mar] Mid-term exam review - En-1004
[21 Mar] Bioinformatics [PDF of presentation] Group 8
[27 Mar] NextGen sequencing [PDF of presentation] Group 7 [see Mason & Elemento 2012]
SMC [22 Mar] Genetics, Evolution, & Language: Origins of Human Diversity [PDF of presentation]

Chapter 18 Quantitative & Behavioural Genetics

[29 Mar] SMC: Quantitative & Behavioural Genetics [PDF of presentation]
[03 Apr] Ainsley Latour on Hamer et al. 1993. A linkage between DNA markers on the X chromosome and male sexual orientation.

Science 261:321-327. [PDF of original] [PDF of presentation]

LOD scores: localizing genes of interest from genomic data

Last updated 02 April 2012
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