2250 - Principles of Genetics - Dr. Carr
Sample exam questions (for Midterms I & II, & Final Exam)
are intended to show the STYLE
of questions that MAY
be asked on exams, NOT the specific questions
that will be asked.
For exams, you will be given a sheet with the Universal Genetic Code: it is therefore not necessary to memorize the genetic code.
I. MOLECULAR BIOLOGY: For each question, indicate in the space provided the LETTER of the ONE response that best answers the question.
In double-stranded RNA
regions, the bonds that hold complementary nucleotides
together are described as
A. ionic B. covalent C. hydrophobic D. hydrogen E. hydrophilic
The teritary structure of protein is determined by
A. beta sheets B. hydrogen bonds C. disulfide bonds D. alpha helices E. subunit arrangement
______ The active site of an
enzyme is most likely
to be composed of amino acids with the following
A. Polar & Hydrophobic B. Polar & Hydrophilic C. Non-polar & Hydrophobic D. Non-polar & Hydrophilic
Semi-conservative DNA replication was demonstrated by
Watson & Crick B. Meselson &
Stahl C. Hershey &
Chase D. Luria & Delbruck
E. Penn & Teller
the template strand of a piece of DNA reads 3'
- AAAAAATTT - 5', its protein product will read:
A. H3N - F F K - COOH B. HOOC - F K K - NH3 C. H3N - K F F - COOH D. HOOC - K K F - NH3
In not more than three grammatically complete sentences, identify and correct the error. Do NOT draw diagrams.Ex.: "DNA probes anneal to particular SNPs and are responsible for genetic diseases."
Ex.: "Met- mutants are caused by a defect of methionine that block its synthesis."Ex.: "Most people do not have Cystic Fibrosis because they do not have the gene for Cystic Fibrosis."
II. MENDELIAN GENETICS. For each question, indicate in the
space provided the LETTER of the ONE response that best answers
Ex: "Brown eyes (B) are dominant to blue eyes (b). Both of my parents have brown eyes, but I have blue eyes. Since eye colour is genetic, I must be adopted."
Sample answer: If eye colour were a simple Mendelian trait, your parents might both be Bb heterozygotes for brown eyes, and you could be a blue-eyed bb homozygote with blue eyes.
woman is heterozygous for an X-linked recessive allele that
causes night-blindness. If she marries a man with
night-blindness, what proportions of her female & male
offspring will carry the night-blindness allele?
A) all, none B) all, half C) half, half D) half, none E) none, half F) none, all
______ In Trufula trees, the locus for leaf colour has three alleles: R (red), G (green), and B (brown). R is dominant to G, G is dominant to B. In the progeny of the cross BR x BG, what fraction will be brown ?
A) All B) 3/4 C) 1/2 D) 1/4 E) 0
______ Two individuals heterozygous for semi-dominant traits
at four loci are crossed: how many F2 genotypes are
A) 4 B) 9 C) 16 D) 27 E) 32 F) 64 G) 81
III. Biotechnology &
each question, indicate in the space provided the LETTER of
the ONE response that best answers the question.
_______Which of the following
could be Type-II restriction endonuclease recognition sites?
A. CGCGCG B. AGCCGA C. AUGCAU D. GGAAGG E. None of the them F. All of them
_______ Oligonucleotides are
described as "sticky." What makes them "sticky" ?
A. They bind to polynucleotides B. They bind to mRNA C. They bind to ddNTPs D. They bind to hnRNA E. They bind to DNA F. Two of these
_______ Southern Blots are
used to detect rare genes. In a Southern blot analysis:
A. DNA probes bind to a base-complementary DNA B. DNA probes bind to an immobilized mRNA
C. RNA probes bind to corresponding cDNA D. RNA probes bind to mRNA
E. Protein binds to corresponding mRNA F. Protein binds to corresponding DNA
_______ A bioinformatic
search for open reading frames will most use which of the
following data base tools?
A. GenBank B. HuGO C. ExPASy D. CanLit E. ENSEMBL F. ORFAnn
IV. GENETIC STRUCTURES: The numbers in the left-hand column refer to structures on the right. For each numbered structure, write the letter of the correct term.V. DNA TRANSCRIPTION & TRANSLATION [Use the Genetic Code table attached to the test]
The following DNA is part of a gene that
codes for a polypeptide of at least seven amino acids:
3' caattgattagtcagtcaattgat 5'
5' gttaactaatcagtcagttaacta 3'
i. Which strand
includes an Open Reading Frame? Top (T) or
ii. Which DNA strand is the sense strand? Top (T) or Bottom (B)
iii. Give the mRNA sequence that would be transcribed from this gene; label the 5' & 3' ends.
iv. Give the seven amino acids that would be translated from the correct message; label the C & N ends.
ANALYSIS: Given the pedigree shown, which of
the six modes of inheritance are possible?
Which is most likely? What observations would
TRI-HYBRID THREE-POINT TEST
CROSS: From the following recombination data,
draw a physical map to indicate the correct order and
distances between loci.
VIII. RESTRICTION MAPPING: From the following fragment size data, draw a restriction map. Label the sites, provide a scale in Kbp.
Watson & Crick received
the Nobel Prize in what year?
(a) 1953 (b) 1962 (c) 1973
Transcription proceeds in which of the following directions (a) 3' 1' (b) 2' 4' (c) 3' 5' (d) 4' 6' (e) 5' 3'
Which of the following amino
acids is/are non-polar?
Draw the structure of Deoxyadenosine monophosphate.
Outline the process of
What are the clues for sex-linked inheritance?
How many introns are present in the chicken ovalbumin gene? (a) 4 (b) 5 (c) 6 (d)
7 (e) more than 7
Text material (c) 2015 by Steven M. Carr