Bio2250 - DNA Open Reading Frame Practice Problems



5'- T A T G G G A T T A T G A T C T C T A A A T G A A A G A T C A C C C C - 3'
3'- A T A C C C T A A T A C T A G A G A T T T A C T T T C T A G T G G G G - 5'


5'- T C A A T G A G G G T C A G G T G A T C A T G T G A C T C C T T C A A T A - 3'
3'- A G T T A C T C C C A G T C C A C T A G T A C A C T G A G G A A G T T A T - 5'


5'- T T G A C T T A G G T A A A A T A A C A A A T T A G T C A T T G G A A - 3'
3'- A A C T G A A T C C A T T T T A T T G T T T A A T C A T T A A C C T T - 5'


3'- c a a t t g a t t a g t c a g t c a a t t g a t - 5'
5'- g t t a a c t a a t c a g t c a g t t a a c t a - 3'


dsDNA practice problems can be generated from the web app RandomORF
          [see Carr et al. 2014 CBE - Life Sciences Education 13,373-374]




The above DNA sequences are from the middle of various eukaryotic protein-coding genes.


a) Identify the single Open Reading Frame in each sequence.

b) Give the polypeptide encoded by that reading frame; label the N and C terminii

c) Is the above process 'Translation' ? Explain.

d) For the advanced student: Can you identify the gene segments above as part of particular protein-coding loci? Use BLAST at NCBI


All text material © 2014 by Steven M. Carr