Bio2250 - Sample Restriction Mapping Problems

In each problem, three restriction endonucleases are used to cut a piece of DNA, singly and in pairwise combination. Sizes of fragments are listed in order of size, not in linear order.
Determine the correct order of restriction sites, and draw the map, with the intervals between sites labelled.



Worked Example

A
)  11, 6, 5

B)  14,8
C)  16,6

A x B) 8, 6, 5, 3
A x C) 11, 5, 5,  1
B x C) 8, 8, 6


D)  18, 5
E)   15, 7, 1
F)   20, 3

D x E)  10, 7, 5, 1
D x F)  15, 5, 3
E x F)   15, 4, 3, 1


G)  14, 1
H) 9, 6
I) 7, 5, 3

G x H) 9, 5, 1
G x I) 7, 5, 2, 1
H x I) 5, 4, 3, 3

Circular Restriction Mapping

A) 17
B) 17
C) 10, 7
A x B ) 12, 5
A x C ) 10, 6, 1
B x C ) 7, 6, 4


All text material 2013 by Steven M. Carr