Bio 2250 -
Sample
Restriction Mapping Problems
In each problem, three
restriction endonucleases are used to cut a piece of DNA,
singly and in pairwise combination. Sizes of fragments are
listed in
order of size, not in
linear
order.
Determine the correct order of
restriction sites, and draw the map, with the intervals between
sites
labelled.
Worked Example
A)
11, 6, 5
B)
14,8
C)
16,6
A
x B) 8, 6, 5, 3
A
x C) 11, 5, 5, 1
B
x C) 8, 8, 6
D)
18,
5
E)
15,
7, 1
F)
20,
3
D x E) 10, 7, 5, 1
D x F) 15, 5, 3
E x F) 15, 4, 3, 1
G)
14,
1
H)
9, 6
I)
7, 5, 3
G x H) 9, 5, 1
G x I) 7, 5, 2, 1
H x I) 5, 4, 3, 3
Circular Restriction
Mapping
A)
17
B)
17
C)
10, 7
A
x B ) 12, 5
A
x C ) 10, 6, 1
B
x C ) 7, 6, 4
All
text
material © 2012 by Steven M.
Carr