Analysis of DNA involves
several aspects of biotechnology:
"The
use of biological processes to produce goods & services"
Forensics
is the use of DNA sequence data to provide legal evidence
Polymerase Chain
Reaction (PCR)
"DNA xeroxing":
in vitro (cell-free) DNA "cloning":
Start with anything with DNA in it: blood, hair, skin, fillet,
etc.
DNA primers copy specific gene
of interest
PCR doubles copy number of gene in each cycle:
12
4
8
16
32
64 etc.: 30 doublings
109
copies [animation]
PCR makes sufficient quantities of purified genes for direct analysis by
...
DNA Sequencing
Automated
DNA sequencing uses laser fluorometry
Modified
ACGTs are attached to fluorescent dyes (A
C G T)
Color-coded bases are incorporated into DNA during copying reaction
DNA sequencer "sees" colours,
"calls sequence" [animation]
"Helix
& Primer" DNA sequencing service at
Memorial University does this locally
DNA Forensics
Applications in fisheries include questions of species identification
& stock structure
1. Same gene has
different DNA sequence in different species
Finding out "Where did this fillet come from?"
Species identification in fish,
seals, deer
, "sea monsters", etc.
2.
Same gene has different DNA sequence in different individuals
of same species
Testing the "Out of Africa"
hypothesis of human origins:
European, Asian, & New World peoples have a common ancestor ~40,000
yrs ago
Testing the "Out of Newfoundland"
hypothesis of harp seal origins:
How do seals move among breeding grounds?
Similar questions can be asked in Atlantic
Cod
DFO / MUN Research Partnership: Marine
Mitochondrial Genomics