ABI377 chromatogram
Chromatogram from an automated DNA sequencer

    The computer scans vertically through each lane of the gel file, and converts the pattern of bands to an individual chromatogram with a series of "peaks" corresponding to each of the bands in the DNA sequence: A C G & TDNA migration slows over the course of the electrophoresis, and multiple bases towards the end may appear as a single broad band instead of discrete "peaks". This phenomenon is similar to the compression that occurs at the top of an autoradiograph. In this experiment, automatic base calling is 100% accurate to position 684, and >98% accurate to the end of the DNA template at 787 bases. Manual editing produces effectively 100% accuracy.

Figure & Text material © 2010 by Steven M. Carr