DNA Sequencing Services and Pricing
GaP uses Applied Biosystems Big Dye chemistry to prepare samples for the ABI 3730 DNA Analyzer (equipped with a 48-capillary array) and ABI 3130 Analyzer (equipped with a 4-capillary array).
||$2.90 per reaction|
|Economy DNA Sequencing
||$3.50 per reaction|
|Full Service DNA Sequencing
||$4.40 per reaction|
For large volume projects (>500 samples/project/year), or if your project does not lend itself to one of the above services, please contact us at the GaP lab to discuss your individual needs.
*Prices are subject to change without prior notice and apply to anyone associated with MUN. Commercial users are invited to contact the facility for quotes.
Sample Submission Guidelines
The quality of DNA sequence is directly related to template quality. With a good template, you can get up to 1000 bases of sequences from a single primer. As an educational facility, we will work with users to ensure that they have the correct amount of clean template and primers according to the following table.
Template Type and Quantity PER 10 uL REACTION
|Single-stranded DNA||50-100 ng||3.2 pmol|
|Double-stranded DNA||200-500 ng||3.2 pmol|
|Cosmid, BAC, etc.||0.5-1.0 µg||5-10 pmol|
- For Economy Sequencing Service, samples and primers must be combined in a 7 uL volume containing 1.6 pmol of primer and the appropriate amount of template (listed in the table above).
- Templates must be in H2O (not TE).
- Users must ensure that plasmids cleaned with mini- or midi-prep kits do not contain any ethanol (hinders the sequencing reaction) and have incubated long enough in water to allow for the homogeneous solution of DNA.
If you wish to submit a sample to the facility, please fill out a Sample Submission Form and a DNA Sequencing Sample List and bring it into the facility with your sample(s). Please contact us if you need any assistance in completing these forms. The GaP facility is located in the Alexander Murray Building, room ER3002.
Turn-around time is approximately 48 hours depending on sample volume at the time. If you require your samples to be analyzed upon receipt, please let us know and we will accommodate your request to the best of our ability. Results can be accessed through the Sequence Data Self-Service Portal. For first time users, please talk to one of us at the facility about having your CREAIT cloud account set up.
GaP runs positive controls with every sequencing run. If we are unable to obtain quality sequence from our control, as well as from your samples, there will be no charge for that run. GaP offers assistance in troubleshooting sample preparation.
Fragment Analysis Services
GaP has considerable experience supporting undergraduate and graduate student projects that require fragment analysis, in addition to designing and executing fragment analysis projects for internal faculty and various government agencies. Due to their complex nature, please contact us to discuss your fragment analysis requirements. We will be happy to assist in project design and execution, and can provide a quote based on your specific needs.
GaP also provides services and training in procedures such as DNA extractions, PCR, PCR amplicon clean-up, running agarose gels, DNA quantification, species identification and sex determination. Please contact the facility regarding fees for these services.
Brettney Pilgrim, M.Sc.
Research Laboratory Coordinator I
Tel: (709) 864-3278