Restriction map of white-tailed deer mitochondrial DNA

    The mitochondrial DNA (mtDNA) molecule of vertebrates is a circular molecule about 17,000 bp (17 Kbp) in circumference. Double-digest analysis of mtDNA from a White-Tailed Deer (Odocoileus virginianus) identifies the positions of 33 six-base-pair restriction sites from 10 different restriction endonucleases (letter codes a, b, c, e, f, h, j, x, y, & z). This gives a restriction map of the molecule based on 198bp, about 1% of the genome.

    The restriction map can be oriented to the functional map of the molecule [below] by the placement of the two Sst II sites [z] at an interval of 1.6kbp between two highly-conserved areas of the 12S &16s rRNA genes.  [SM Carr et al. 1986. Proc Natl Acad Aci 83:9576]