Double-Digest Restriction Mapping

    A PCR-amplified  DNA fragment has been digested with three different restriction endonucleases (EcoRI, Bgl II, & MboI), singly (lanes 1-3) and in pairwise combination (lanes 4-6). Comparison with a molecular weight standard (lane 7) allows a determination of the sizes of the fragments in the digests, which in turn permit an inference of the order and distances among restriction sites in the DNA fragment. The logic of restriction mapping will be explained in Laboratory.

All figure & text material © 2008 by Steven M. Carr