Fluorsecent In Situ Hybridization (FISH)

    DNA probes specific to regions of Individual chromosomes may be tagged with fluorescent markers and hybridized with a chromosome spread. In this computer-enhanced image, chromosome spreads from a single individual are identified sequentially by the use of a series of probes tagged with the same fluorchrome. In the amplified view at right, male and female karyotypes are readily distinguished by hybridization with X- and Y-specific probes. By adjusting the fluorescence wavelength of the different tags, chromosomes may also be identified simultaneously by the technique of Chromosome Painting.

Figures ©1996 by Griffiths et al.; all text material © 2007 by Steven M. Carr