Our laboratory utilizes the sea urchin embryo as a paradigm of embryonic development. During the past several years, we have investigated the role of the extracellular matrix in the development of this embryo. Most of our efforts have been directed at the apical ECM, the hyaline layer. We have utilized a range of methodologies to identify, characterize and functionally define the various protein components of the hyaline layer. We have also purified and characterized serine and metallo- proteases which utilize constituents of the layer as substrates. We now possess a detailed picture of the composition and role of the hyaline layer during development of the sea urchin embryo.

More recently, we have embarked on a project to biochemically characterize the major yolk protein, MYP, present in the yolk granule and coelomic fluid of the sea urchin. Our emphasis is on defining the functional capabilities of this protein as well as identifying differences in the roles played by the egg and coelomic fluid forms of the MYP.