Schematic strategy for whole-mtDNA-genome PCR amplification & sequencing
Schematic strategy for whole-mtDNA-genome PCR amplification & sequencing
Red boxes
are short L-strand (5'
3') PCR primers;
blue boxes are primers for the reverse complement
H-strand. Sixteen such primer pairs amplify the entire mtDNA molecule as
an ordered series of sixteen overlapping ~1Kbp contigs (longer red- &
blue-tipped boxes). Forward- and reverse-sequencing of all products in
the set produces the complete mtDNA genome sequence for an individual.
The strategy
differs substantially from conventional genomic sequencing based
on random ("shotgun") sequence assembly