Microsatellites by Capillary Electrophoresis

Microsatellite profiles detected by multiplex capillary electrophoresis

Microsatellites can be fluorescently tagged with the same dyes used in DNA sequencing. With 16 loci [boxes in top panel] spread over four labeling reactions, each with a separate color [blue, green, yellow (shown in black), & red] [bars in middle panel], all 16 loci can be combined in a single multiplex experiment [bottom panel] Allelic variants at each locus are identifiable as a particular color over the size range specific to the locus.