
Primer design for Polymerase Chair Reaction
(PCR) amplification of Anarhichas mtDNA genomes
The 16 Kbp mitochondrial DNA genome of Anarhichas
spp. was initially amplified with three PCR Primer
pairs in overlapping regions of 4.3,
4.6, and 6.8 Kbp. Each region was then
sequenced with a series of short overlapping internal primers
that covered each region in blocks of ca. 300 ~ 400 bp.