Biol2250 – Final Exam questions
Spring 2024Dr Carr
For each of the following questions, write a
500 ~ 750 word essay. The essay should show your understanding
of the lecture material, demonstrate that you have prepared the
answers ahead of time, and wherever possible the use of
outside materials.
For the exam, I will
select FOUR of the questions, and you must answer any THREE.
I sometimes require that one question in particular be
answered, and then you have your choice of any TWO of
the others.
I want
you answer all questions, in detail, as part of
the learning experience. The Exam then confirms that you did the
assignment. 1.Distinguish
between Genetics, Molecular Biology, and Biotechnology.
Illustrate the differences by citing one example of each, and
showing the degree to which the example fits best into one type
of science, and not so much the others.
2. What is a "Gene"? Compare and
contrast notions of the eukaryotic gene after "cracking" of
the Genetic Codein 1965 with contemporary
notions in 2024. 3.I have described DNA
replication as “DNA xeroxing”, and RNA
transcription from DNA as making a “Silly
Putty” copy. In what ways are these descriptions accurate?
In what ways are these descriptions inaccurate?
4. Explain how different kinds of SNP variation
may alter the DNA sequences of genes, and the
consequences for organisms.
5. Describe the process of obtaining a DNA
sequence of a particular gene, with emphasis on the
biochemistry of DNA extraction, PCR amplification,
and automated "Sanger" DNA sequencing.
6. What are the most important parameters in
designing oligonucleotide primers for PCR?
7. From the accompanying data, construct a Restriction
Map of the target DNA.