Apis mellifera ssp PCR primers
The two primers span the Cox1
and Cox2 regions of the Apis
mellifera mtDNA genome. The primer
sequences are given, the number of bases of each type
and their molecular weights (MW), the total formula
weight (FW) of the primers, and the number
of nanomoles (nmoles) synthesized, as
calculated from the Optical Density (OD).
Given these values, calculate the ul of
H20 to be added to each tube to create a
stock solution of 100 uM. Include this information in
your notebook. Show your work.
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2024 by Steven M. Carr