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Mass Spectrometry

The facility has five mass spectrometers with a number of sample introduction options, ionization sources and analyzer types to allow analysis over a large mass range from small molecules to large proteins. There are two LC/MSD instruments (Agilent) with the option of a quadrupole or ion trap analyzer and the ability to run in LC standalone mode with UV-VIS detection. A high resolution MS (Waters) and a MALDI (Applied Biosystems) have TOF analyzers providing high sensitivity and resolution.

Potential users of the facility are encouraged to meet with the operator prior to training to discuss the scope and nature of their work to determine the most appropriate instrument choice. Access to the lab is limited to 9:00am to 5:00pm Monday to Friday. More experienced users may request 24/7 access.

LC/MSD (TOF) Agilent 6200 series Accurate- Mass-Time- of- Flight

• HPLC ( 1260 Infinity) interfaced to a 6230 TOF MS (an orthogonal time-of-flight analyzer)
• HPLC equipped with vacuum degasser, quaternary pump, autosampler and variable wavelength detector (190-600nm)
• MS has Dual Electrospray (dual-ESI) and Atmospheric Pressure Photoionization ( APPI) chambers
• Ion detection in positive , negative and fast polarity switching modes
• Software: MassHunter Workstation version B.05.01

LC/MSD (Trap) Agilent 1100 series SL

  • HPLC ( 1100 series) interfaced to an ion trap mass analyzer ( model G2445D SL)
  • LC equipped with a vacuum degasser, quaternary pump, thermostatted auto sampler and UV-VIS diode array detector ( wavelength range 190-900nm)
  • MS has Electrospray (ESI) and Atmospheric Pressure Ionization (APCI)
  • MS has an ion trap analyzer with mass range 50-2200 Da with adjustable scan speeds for normal,enhanced or maximum resolution and extended range 50-4000 Da with normal resolution
  • Scan, Multiple Ion Monitoring (MRM) and MS(n) modes with positive, negative and alternating polarity ion detection
  • Sample introduction via LC or syringe pump for direct infusion experiments and optimization
  • Software: ChemStation for LC 3D Rev.A.10.02 [1757] , Bruker MSD Trap Control Version 5.2 Build No. 63.8

GCMS Agilent

  • GC (model 6890N) interfaced to a single quadrupole 5973 inert MSD (model G2578A)
  • GC has a split/splitless injector with electronic pneumatic control and autosampler
  • MS has a quadrupole mass analyzer with a mass range of 1.6-800 Da
  • SIM( single ion monitoring ) and scan mode available with positive ion detection
  • Software: MSD ChemStation D.01.00 Build 75

High Resolution MSD Waters Micromass GCT Premier

  • MS has a high-performance, orthogonal acceleration time-of-flight (oa-TOF) mass analyzer allowing fa st acquisition of full spectra with high sensitivity, mass accuracy and resolution
  • Sample introduction via GC (Agilent 6890N) for volatile compounds or Direct Insertion (DI) probe for non-volatile compounds
  • EI (electron impact) and CI (chemical ionization) modes available with positive and negative ion detection
  • Mass range of 4000 Da
  • Mass accuracy between measured and calculated mass is
  • Software: MassLynx V4.1 : SCN 569

4800 MALDI TOF/TOF Applied Biosystems/MDS SCIEX

  • MALDI (matrix assisted laser desorption ionization) instrument features tandem TOF (time-of -flight) analyzers
  • Variable ion path length to accommodate MS linear, MS reflector and MS/MS modes
  • Nd:YAG laser operates at 355nm to ionize samples
  • positive and negative ion detection
  • High-resolution precursor selection (MS/MS mode)
  • Automated single-plate sample-loading system
  • Software: 4000 Series Explorer V3.5.3 ( Build 1017)