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Proteomics

Lidan

The facility provides proteome-analysis, including protein identification, the identification of protein post-translational modifications, peptide de novo sequencing, protein quantitation and metabolite identification.

Instrumentation:

Qstar XL LC/MS/MS System

The ABI Qstar XL Hybrid LC/MS/MS system is a quadrupole time-of-flight system with high sensitivity and excellent mass accuracy. It is equipped with a NanoSpray ion source, an IonSpray ion sourse and an APCI heated neubulizer ion source. There is an oMALDI 2 ion source available and a ProBot fraction collector allowing automated MALDI plate spotting from the nanoLC.

Available data processing software includes BioAnalyst, ProID, Pro ICAT, Pro BLAST, Pro QUANT, Metabolite ID and MASCOT and there is a fast MASCOT database server on site.

DIONEX UltiMate 3000 Nano and Capillary LC System

A DIONEX UltiMate 3000 LC separation system is interfaced with the QSTAR XL. The combination enables LC-MS, LC-MS/MS and LC/LC-MS/MS analysis of complex protein mixtures.

Sample Submission:

If you wish to submit a sample to the facility, please fill out a sample submission form (Fillable PDF formate) and bring it in with your sample. Any handling precautions and special safety concerns for your sample should be indicated on the sample submission form.

Proper sample preparation is extremely important for a mass spectrometry analysis. In order to maximize the likihood of a successful analysis, we recommend you to read our sample preparation Guidelines. If you have any questions about sample preparation, analysis method and data interpretation, please fee free to contact us.

Fee:

User fee details. The rate is subjected to change and based on the supplies for running the samples, instrument maintenance and instrument usage. For off-campus users, please contact us to request the quotes.

Contact Information:

Lidan Tao
ER3002 (Lab) and ER3005 (Office)
Earth Sciences, Alexander Murray Building
Memorial University of Newfoundland
St. John's, NL, Canada, A1B 3X5
Tel: (709) 864-3738
email: ltao@mun.ca