The Epifluorescence Microscope
Epifuorescence microscopy is a method of fluorescence microscopy that is widely used in medical and biological sciences. The excitatory light passed from above, through the objective and then onto the specimen instead of passing it first through the specimen. The fluorescence in the specimen gives rise to emitted light which is focused to the detector by the same objective that is used for the excitation. A filter between the objective and the detector filters out the excitation light from fluorescent light. Since most of the excitatory light is transmitted through the specimen, only reflected excitatory light reaches the objective together with the emitted light and this method therefore gives an improved signal to noise ratio. A common use in biology is to appy fluorescent or fluorochrome stains to the specimen in order to image a protein or other molecule of interest.
Nikon's Eclipse E600 research microscope is equipped with the revolutionary CF160 infinity optical system, providing bright, sharp, crisp and clear images in all applications.
Our Eclipse E600 epifluorescence microscope is equipped with 3 filter blocks (B-2A, UV-2A and G-2A). The universal objectives (4X, 10X, 20X, 40X and 100X) allow for multiple observation techniques--such as brightfield, darkfield, Nomarski DIC, epi-fluorescence or phase contrast--eliminating the need to change objectives while maintaining the same optical quality as dedicated lenses. The light source for the Epifluorescence is super high-pressure mercury lamp.
The digital imaging system is a Leica DFC500 digital camera.
