Aquatic Health Assays

We offer research support with assays for use in Environmental Effects Monitoring (EEM), fate and effects studies, aquaculture research and other areas where toxic, pharmaceutical or other health impacts may be seen in fish. These tests are of use to scientists in the gas and oil, mineral exploration, environmental and fisheries and aquaculture fields. They are available to academic, government and industrial users.

We are happy to work to implement new assays that may be of use to you in your research. Please contact the facility manager for more information.

Trout blood cellsHaematology. We can perform basic haematological work including blood smears and differential white blood cell (WBC) counts. Imbalances in WBC counts of neutrophils, thrombocytes and lymphocytes may be associated with disease in fish.
GillPathology. Stained slides can be prepared from fixed animal tissue for a variety of uses. This may be provided to your veterinary pathologist for clinical evaluation. We are able to perform basic pathological assessments of gill tissue. Gill tissues often show abnormalities as a result of chemical or pathogenic exposure.
Microwell plateMixed Function Oxygenase (MFO). Mixed function oxygenase (MFO) enzymes are involved in a range of metabolic activities in response to endogenous and exogenous compounds. In fish MFO activity is induced by exposure to toxins. In this assay, EROD (ethoxyresorufin-o-deethylase) activity is measured.
PhagocytosisPhagocytosis assay. In vitro measurement of the phagocytic ability of macropage iosolated from fish can give an indication of enhancement or imparement of immune activity. This can be used to assess the impacts of pharmaceuticals, feed, or environmental toxins on fish immunity. 
Oh No phagoOxidative burst assay. This assay may be done in conjunction with the phagocytosis assay. The oxidative burst response of phagocytic cells produces superoxide anions as part of an immune response. This response may be altered by disease or pharmaceuticals. 

Contact

Ocean Sciences

230 Elizabeth Ave, St. John's, NL, CANADA, A1B 3X9

Postal Address: P.O. Box 4200, St. John's, NL, CANADA, A1C 5S7

Tel: (709) 864-8000