Mass Spectrometry
Matrix-Assisted Laser Desorption-Time of Flight Mass Spectrometer (MALDI-ToF MS)
The instrument is an Applied Biosystems DE-RP equipped with a reflectron, delayed ion extraction and high performance nitrogen laser (337 nm). Both positive or negative detection is available. In the linear mode the upper mass range is 350 kDa with a resolution of 1,000 (for m/z 17,000). In the reflector mode the upper mass range is 6,000 Da with monoisotopic resolution of 10,000 (for m/z 5,700). MS/MS experiments can be performed by P ost S ource D ecay (PSD).
Atmospheric Pressure Ionization-Mass Spectrometer (API-MS)
This mass spectrometer is part of an Agilent 1100 series LC/MSD chromatographic system. In the FIA mode (flow injection analysis) sample solutions are injected into a quadropole mass analyzer with a mass range of m/z 50-3000 and a mass accuracy of 0.1 amu. The MS can perform either electrospray (ESI) or atmospheric pressure chemical ionization (APCI) and positive or negative ions can be acquired. The computer has de-convolution software for multiply- charged ions.
These instruments were purchased with financial support from The Canadian Foundation for Innovation.