The ArkChip - A multi-species, iterative mtDNA sequencing strategy for biodiversity and phylogeographic genomics.
(SM Carr, HD Marshall, AT Duggan, SMC Flynn, KA Johnstone, AM Pope, & CD Wilkerson 2008. Comp. Biochem. Physiol., D: Genomics and Proteomics 3,1-11.)
The current generation of DNA re-sequencing
microarrays accommodates >120Kbp, enough to
include seven
species' complete mtDNA
genomes. Since the 25b oligonucleotides are
species-specific,
re-sequencing of all included genomes can be accomplished
simultaneously. Separate
investigations on distinct species can be "multi-plexed." The figure
shows a four-species experiment with two fish (Gadus & Anarhichas) and two mammals (Pagophilus & Rangifer),
applied singly (top two panels), or in pairwise or four-fold patterns
[bottom two panels]. Note that the binding is species-specific, with
short regions of cross-species hybridization (<4%) for regions
conserved between vertebrtes species (e.g., rRNA regions).
Material © 2008 by Steven M. Carr