Recombination at the Molecular Level IV


After branch migration has proceeded for some distance, the junction point is again nicked. The two DNA molecules separate, and the nicks are ligated. Each DNA molecule now combines an end from one chromosome with the other end of its homologue. The intermediate dsDNA is a heteroduplex that includes a region in which one strand comes from one molecule and one from the other. If nicking, branch migration, and re-ligation has been resolved between the two loci, their respective markers will recombine : in the example, AB // ab  becomes Ab // aB.

All text material ©2014 by Steven M. Carr