Gel electrophoretic
detection
of RFLP variation
SNPs (single-nucleotide
polymorphisms) that create or destroy restriction sites lead to RFLPs (restriction
fragment length polymorphisms) detectable by gel
electrophoresis. The
outside lanes are molecular size standards. Lanes 2 & 5 show the
typical pattern of a
single
8 Kbp fragment. Lanes 1 & 3 show two variants that result from the
presence of an additional restriction site. Lane 4 shows a variant
of the genotype in Lane 3, due to an extra restriction site. [Note that
the
restriction
maps shown at right cannot be reconstructed from the gel data shown,
but
could be mapped with reference to data from other restriction enzymes].