Gel electrophoretic detection of RFLP variation

    SNPs (single-nucleotide polymorphisms) that create or destroy restriction sites lead to RFLPs (restriction fragment length polymorphisms) detectable by gel electrophoresis. The outside lanes are molecular size standards. Lanes 2 & 5 show the typical pattern of a single 8 Kbp fragment. Lanes 1 & 3 show two variants that result from the presence of an additional restriction site. Lane 4 shows a variant of the genotype in Lane 3, due to an extra restriction site. [Note that the restriction maps shown at right cannot be reconstructed from the gel data shown, but could be mapped with reference to data from other restriction enzymes].


All text material ©2014 by Steven M. Carr