Use of an RFLP as a co-dominant genetic marker for a recessive trait
In the pedigree,
birth of individual II-2
with the recessive phenotype indicates that the parents must
heterozygotes: the other unaffected sibs seek genetic counseling
determine if they may be carriers. The genetic
shows that two (II-3 & II-4) are carriers,
of the two children of the latter (III-2)
also a carrier.
same experiment were done by amplifying the 5kb region by
PCR, and the products
with the restriction enzyme and separated
by electrophoresis, and that the mutation
occurs 2.5 kb from the middle restriction
site and 0.5 kb from the right-hand restriction site. How many bands would you
see in the heterozygote?
Draw the expected gel
result. Is this result the
same or different than the
previous homework assignment? Explain.