Consequences of mutations in the upstream promoter region
The effect of a
mutation at each
can be measured by comparing the level of mRNA
to the unmutated gene [Vertical scale]. The graph shows strong reduction of transcription
by mutations in the promoter
regions at -10
& -35 (GGCCAATC),
and at an
upstream enhancer region.
Note that mutations at two sites immediately 5'
the -35 promoter sharply increase
transcription. A mutation at the start point of
turns off transcription entirely.
Note that the majority of mutations in this region have no measurable affect on transcription. Dots indicate positions where the consequences of mutations are unknown.