M
N M A L T L F T
N T A L A S L L V
L I A F W L P Q L
PHOCND3
ATAAACATAGCACTAACCCTATTTACCAACACAGCCCTGGCCTCTCTACTCGTACTAATTGCATTCTGACTCCCTCAGCT
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HALIND3
ATAAACATAGCATTAACCCTATTTACCAACACAGCCCTAGCCTCTCTACTCGTACTAATTGCATTCTGACTCCCTCAGCT
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CANIND3
ATAAACGTTATATTAACTTTGATAACTAATGTAACCCTAGCATCCTTACTTGTACTAATCGCATTCTGACTTCCCCAGCT
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FELIND3
ATAAATGTAATACTTGCCTTACTTACCAATACACTCCTGTCCACACTACTTGTACTCATCGCATTCTGATTACCCCAACT
"Universal" PCR primer design in the mtDNA ND3 region
The diagram shows
part of the DNA sequences of
the
ND3
subunit gene
from the mitochondrial DNA of
four species of mammalian Carnivora,
harbour seal (Phoca), grey seal (Halichoerus), domestic
dog (Canis), and domestic cat (Felis).
The
nucleotide region shown
in colour is a potential PCR primer
site, a region of >25
bp region that is identical
between
seal species sequences and >92%
identity
with the canid and / or felid sequences. This primer, paired with
another a few hundred or few thousand bases away, will likely amplify
the gene region for most species of Carnivora.