Inter-specific RFLP variation
of deer mitochondrial DNA
The restriction map
of
a white-tailed
deer (Odocoileus virginianus) from Savannah
River, South
Carolina,
includes
33 restriction sites from 10 different restriction
endonucleases
(letters
a,
b,
c, e, f, h, j,
x,
y, &
z). Comparison with the restriction map
of a black-tailed
deer (O.
hemionus columbianus) from Hopland,
California,
shows 20 restriction site polymorphisms. There are nine
additional sites present
(letters
above the line)and 11 sites absent (letters
below
the line) in comparison with the white-tailed deer restriction
map. Gain
or
loss
of
a
restriction site is due to the mutation of a single nucleotide
in each
site (SNP variation).
Comparison of the fraction of sites shared between maps
therefore
permits an estimate of the DNA sequence divergence between
the
two
species. [Carr
et al. 1986. Proc Natl Acad Aci 83:9576]