Microsatellite autoradiogram

    Microsatellites in the CODIS set were originally run as radioactively-labeled PCR products in a single gel electrophoresis experiment. In an autoradiogram where all loci are shades of grey, bands corresponding to alleles attributable to different microsatellite loci, but with similar molecular weights, can be difficult to distinguish. Compare this with a fluorescently-labeled, capillary electrophoresis profile, where locus-specific color patterns allow automation of the identification.

Figure & text ©2016 by Steven M. Carr