Microsatellite autoradiogram
Microsatellites in the CODIS set were
originally run as radioactively-labeled
PCR products in a single gel electrophoresis
experiment. In an autoradiogram where all
loci are shades of grey, bands attributable to different
microsatellite loci, but with similar molecular weights, can
be difficult to distinguish. [Compare this with a fluorescently-labeled,
capillary-electrophoresis profile].
HOMEWORK: In this experiment, suppose that blood at a murder scene has been typed with four loci from the CODIS set [middle lane], and compared with six forensic samples [three adjacent lanes on either side]. (1) Ascertain which (if any) of the forensic samples is a match.(2) One of the samples is from the son of the murder victim. Which one? How do you know? (3) Could you distinguish this sample from one from the brother of the victim? Explain.
HOMEWORK: In this experiment, suppose that blood at a murder scene has been typed with four loci from the CODIS set [middle lane], and compared with six forensic samples [three adjacent lanes on either side]. (1) Ascertain which (if any) of the forensic samples is a match.(2) One of the samples is from the son of the murder victim. Which one? How do you know? (3) Could you distinguish this sample from one from the brother of the victim? Explain.