Multiplex detection of microsatellite variation by capillary gel electrophoresis
The 13 microsatellite
loci in the CODIS set include thee subsets that
vary over distinct
ranges of base pairs, such that it is possible to
combine multiple microsatellites for analysis in a
single multiplex electrophoresis experiment. In
the example, four of the markers show allelic variation
occuring in four non-overlapping ranges between 90 ~ 360
bp. Note that at each locus, the individual tested shows
two prominent peaks corresponding to two alleles
(heterozygous), as expected for highly
polylmorphic marker. In some cases, an individual may be
homozygous for a single allele, in which case
only a single peak would be seen.
Figure © 2012 TA Brown, Introduction to Genetics (1st ed.); additional text © 2012 by Steven M. Carr