Multiplex detection of microsatellite variation by capillary gel electrophoresis
The 13
microsatellite loci in the CODIS set include
thee subsets that vary over distinct ranges of base pairs, such that
it is possible to combine multiple microsatellites for
analysis in a single multiplex electrophoresis
experiment. In the example, four of the markers show
allelic variation occuring in four non-overlapping
ranges between 90 ~ 360 bp. Note that at each locus, the
individual tested shows two prominent peaks
corresponding to two alleles (heterozygous),
as expected for highly polymorphic marker. In other
cases, an individual may be homozygous for a
single allele, in which case only a single peak would be
seen.