Microsatellite profiles detected by multiplex capillary electrophoresis

    Microsatellites can be fluorescently tagged with the same dyes used in DNA sequencing. With 13 loci in the CODIS set spread over three labeling reactions, each with a separate color [blue, green & yellow [shown in black]], all 13 loci can be combined in a single multiplex experiment [fourth box]. with the remaining color [red] used as a size standard [red bands]. Allelic variants at each locus are identifiable as a particular color over a particular size range.

Figure ©2012 TA Brown, Introduction to Genetics (1st ed.); additional text ©2016 by Steven M. Carr