Common Chemical Mutagens

5-Bromouracil is used in cytogenetic studies to label newly-synthesized chromatids in order to show recombination events. Nitrous Acid is produced from Nitric Acid, a common food additive, by the action of stomach acids. EMS can be fed directly to Drosophila and other organisms to induce mutations. Ethidium Bromide is commonly used as a fluorescent stain of DNA. Details of mutagenesis are given in lecture.

Figure 2012 TA Brown, Introduction to Genetics (1st ed.); additional text 2012 by Steven M. Carr