RNA transcription proceeds 5'3'

Note that the DNA template for transcription is oriented in the antiparallel direction with respect to the new RNA molecule. RNA Synthesis takes places 5'3'.

DO NOT think of this process as 'DNA is read 3' to 5'.

Figure © 2012 TA Brown, Introduction to Genetics (1st ed.); additional text © 2014 by Steven M. Carr