The Hershey & Chase Blendor Experiment (Part 2)

    During the process of infection of bacteria by phage, a quick spin in a Waring blendor shears phage away from the outside of the bacteria. Analysis of the bacterial pellet and supernatant fractions demonstrates that 32P phosphorus-labelled DNA inside the phage shows up inside bacteria (left), whereas the 35S sulfur-labelled protein in the external coat remains in the supernatant (right). Further, phage labelled with 32P go on to produce progeny phage that are also labelled. This biological experiment confirms Avery's conclusion that DNA is the transforming substance, and shows that transformation is inherited.

HOMEWORK: The Hershey & Chase blendor experiment is often described as "quick & dirty": Why? Explain, in terms of  the key figure in Hershey & Chase (1952).

All text material ©2016 by Steven M. Carr