Chain - termination DNA
sequencing
Replication of the DNA
strand at
the top with a reaction mixture of standard dNTPs and one ddNTP
(here, ddATP) produces a set of DNA
fragments in which chain growth has been terminated at each position
where
a ddA has been incorporated.
Four
separate
reactions with each of the four ddNTPs produces a complete set
of
fragments that differ by plus or minus one nucleotide.