Linearization of a circular DNA vector

A circular plasmid DNA molecule cut at one of the endonuclease restriction sites in its polylinker is transformed into a linear molecule with single-stranded "sticky ends." In this case, digestion with EcoRI leaves ~~TTAA-5' overhanging ends.

Figure © 2000 by Griffiths et al. ; text © 2010 by Steven M. Carr