Experiment 7:  Progeny of S35-Labelled Phage  Nearly Free from the Parental Label


Lysates of Bacteris Infected with S35-labelled T2 and stripped in the Waring blendor

Percent of adsorbed S35 or of phage yield

Cells Stripped

Cells not stripped

 

S35

Phage

S35

Phage

Eluted in Bendor fluid

86

-

39

-

1st low speed sediment

3.8

9.3

31

13

2nd low speed sediment

0.2

11

2.7

11

High speed sediment

0.7

58

9.4

89

High speed supernatant

2.0

1.1

1.7

1.6

Recovery

93

79

84

115

 

 In this experiment Hershey and Chase infected bacterium with 5 S35 labelled T2 phage each.  The bacteria were separated from the unadsorbed phage and suspended in a solution of 1Mm MgSO4, .1mM CaCl2 and .1gm gelatine.  One sample of this suspension was run in a Waring blendor for 2.5 minutes and centrifuged to remove any extracellular S35.  Another sample was taken and centrifuged, but not run in the blendor.  These different samples are indicated above: Stripped= run in blendor, not stripped=not run in blendor.  The cultures were lysed by adding .002mM HCN, n X 10^11 UV killed T2 and .6mg NaCl per mL of culture.

 The data above was collected by fractionating and assaying the lysates.  If the amount of S35 is compared between the two samples it is obvious that the majority of it is removed in the stripped sample.  The sediment with the most progeny has almost 10% S35 if the cells are not stripped of the phage coats and less then 1% if the coats are removed.  This shows that the majority of the S35, by far, is present in the coats of the T2 phage and very little of it, if any, is passed on to the progeny.