Host transformation by Plasmid integration

    Exposure of antibiotic-sensitive E. coli cells to the recombinant plasmid allows integration of the plasmid DNA into the bacterial DNA (transformation).  The bacterial cells then take on the genetic characteristics of the plasmid, which in a cloning experiment  include resistance to antibiotics. Growth of individual bacterial colonies on media with antibiotics then indicates cells that have successfully integrated plasmid DNA along with the cloned insert DNA


All text material 2013 by Steven M. Carr