Recombination of "Sticky Ends"
DNA molecules from two different
sources (blue and
cut with the same restriction
endonuclease enzyme, in
EcoRI. This leaves fragments with
overhanging ~~TTAA-5' "sticky
ends". Because the EcoRI
site is a palindrome, tails from the blue
and red DNAs are
complementary and can come together in new combinations
in the sticky ends are sealed with the enzyme DNA ligase.