A cDNA
Microarray
("GeneChip"):
measuring variation in RNA expression
A cDNA
Microarray
("GeneChip"):
measuring variation in RNA expression
This array
includes
1,046 different DNA
oligonucleotides, each specific for a particular cDNA
sequence generated from the mRNA
transcriptome of a particular tissue.
The cDNAs are
fluorescently
labelled and applied to the chip. Quantitative patterns of
gene
activity are indicated
by pseudocolours:
high levels
of binding (and thus high levels of mRNA
expression) are
indicated
by "hot" or
"warm"
signals,
while
"cool" and "cold" signals indicate
low
expression.