Contig RFLP Mapping

    A series of cloned DNA fragments (1 - 5) are individually mapped with the same set of restriction endonucleases (B = BamHI, F = FnuDII, N = NotI, & S = SalI). Comparison of the order and distances among restriction sites at the left and right ends shows how they overlap with each other.  Assembly of these adjacent, overlapping ("contiguous") maps allows a complete restriction map to be assembled for the entire region (bottom line).

    Note the similarity between this procedure and a the three-point test cross: both are used to determine the order and distances among genetic markers. The DNA map is of course at a much smaller scale.

All text material © 2012 by Steven M. Carr