Contig RFLP Mapping
A series of cloned
(1 - 5) are individually mapped with the same set of
(B = BamHI, F = FnuDII, N =
S = SalI). Comparison of the order and distances
among restriction sites at the left and right ends shows how they
overlap with each other. Assembly of these adjacent,
maps allows a complete restriction map to be
assembled for the entire region (bottom line).
Note the similarity
between this procedure and a the three-point
cross: both are used to determine the order and
distances among genetic markers. The DNA map is of
course at a much smaller scale.