Bio4241 -
Comprehensive exam questions, for Wednesday, 07 April 2010, 2-4P
Instructions: Prepare answers for
each of the following questions: your answer for each should fit on the
front and back of no more than two lined
sheets
of paper. For the exam, I will select
at random five questions.
You
will answer any three
questions;
you may not answer a question pertaining to your own
group's presentation.
You may bring notes or outlines of your prepared answers, on the front
and back of one 8.5 x 11 sheet of paper. [Please don't try to cram complete essays
onto this page!]. You will have two hours to answer the four questions.
What is a Gene:
How would you define the concept "gene"
in
2010?
Compare and contrast your definition with the gene as it was
understood by the time of the “cracking of the genetic code” ca. 1965,
and the classical notion of "gene" as it was before Watson & Crick
(1953).
Chap.
7:
Answer Question #11 on the mapping of
bacterial chromosomes (p. 209 in MGA2)
Chap. 12: Explain the difference between "Forward"
& "Reverse" genetics.
Describe how each approach would be applied to understanding the nature
of wrinkled
vs. round peas. (cf. discussion of Alkaptonuria,
pp. 240-241)
Chap.
13: For each of the four lac-operon genotype classes in the
table below,
explain the associated phenotypic pattern of B-Galactosidase activity,
in the presence or absence of lactose.
Chap. 15: Answer Question #21
(p. 508), concerning the genetics of Retinoblastoma.
Chap. 16: Explain the genetic basis of the banding pattern
in Figure 16-25 (b) (p. 536) ;
include a discussion of the technical means by which the colored bands
were produced
Bioinformatics: Choose
any
one of
the following NGS methods:
explain the principle at the level of a Bio2250 class lecture
Roche/454
FLX
Pyrosequencer (www.454.com)
Illumina
Genome
Analyzer (www.illumina.com)
Applied
Biosystems
SOLiD
Sequencer (marketing.appliedbiosystems.com)
Chap
18: Quantitative Genetics:
In Figure 18-7 (p. 595), Explain the range of phenotypic response of Drosophila to different
temperatures, in terms of the Norm of
Reaction