³²P-labelling of DNA

    For use in autoradiography, a radioactively-labelled dNTP molecule is synthesized with the first ("alpha") PO₄ containing radioactive ³²P rather than non-radioactive ³³P. During synthesis of the new DNA strand, the outer two PO₄ groups are cleaved off and the labelled PO₄ is incorporated into the DNA backbone.

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All text material © 2009 by Steven M. Carr

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