32P-labelling of DNA

    For use in autoradiography, a radioactively-labelled dNTP molecule is synthesized with the first ("alpha") PO4 containing radioactive 32P rather than non-radioactive 33P. During synthesis of the new DNA strand, the outer two PO4 groups are cleaved off and the labelled PO4 is incorporated into the DNA backbone.

All text material © 2013 by Steven M. Carr