32P-labelling of DNA

    For use in autoradiography, a radioactively-labelled dNTP molecule is synthesized with the first ("alpha") PO4 containing radioactive 32P rather than non-radioactive 33P. During synthesis of the new DNA strand, the outer two PO4 groups are cleaved off and the labelled PO4 is incorporated into the DNA backbone.

All text material 2013 by Steven M. Carr