detection of RFLPs
The Southern Blot autoradiogram show that a
DNA fragment with a sequence homologous to the probe DNA
made from clone #10 is present in clones ## 3, 4,
& 8, with the expected size of 400bp, and absent
in clones ##1 ,2, 5, & 9. [In the control experiment,
the probe sticks to itself in lane 10]. In lane #6, a
homologous sequence is present, but as two bands, of sizes 300bp
and 100bp. This indicates that the homologous DNA is
present, but has an additional restriction site that cuts
the fragment into two smaller fragments, 100bp from one end.
Thus, clone #6 shows a restriction
length polymorphism (RFLP),
indicative of DNA sequence
variation among the clones. The right-hand panel show the
autoradiogram in schematic form.
modified from (c) 2000 by Griffiths et al.; text
material (c) 20136by Steven M.