The Southern Blot
autoradiogram show
that
a
DNA fragment with a sequence homologous to the probe DNA
made from clone
#10 is present in clones ## 3, 4, & 8, with the expected
size of 400bp,
and absent in clones ##1 ,2, 5, & 9. [In the control
experiment, the probe sticks to
itself
in lane 10]. In lane #6, a homologous sequence is present, but
as two bands, of sizes 300bp and 100bp. This indicates that the
homologous
DNA is present, but has an additional restriction site
that
cuts the fragment into two smaller fragements, 100bp from one
end.
Thus, clone #6 shows a restriction
fragment length polymorphism
(RFLP), indicative of DNA sequence variation among the
clones. The
right-hand panel show the autoradiogram in schematic form.
