Bay Bulls, Newfoundland Spring 2000
(D. Innes)
Dr. David J. Innes
Department of Biology
Memorial University of Newfoundland
St. John's, Newfoundland, A1B 3X9, Canada
Phone: (709) 864-4754 Fax: (709) 864-3018
email: dinnes"at"mun.ca (replace
at with @)
Research
The research in my laboratory focuses on sexual and asexual
reproduction, primarily using the planktonic
crustacean, Daphnia pulex as a model organism
(visit the Cladoceran
Web Site at the University of Guelph). We are also investigating the
evolutionary genetics of the interaction between the mussels Mytilus edulis, M.
trossulus and their hybrids in Newfoundland (in
collaboration with Dr. Ray Thompson, Ocean Sciences Centre). Collaborative
research has been carried out with Dr. Luise Hermanutz (Biology, MUN) on population and reproductive
ecology of moss campion (Silene
acaulis) and bog bean (Menyanthes
trifoliata) in Newfoundland, and the reproductive
ecology of Grevillea spp. in NSW Australia.
Research on the population structure and mating systems in Newfoundland spiders
has been conducted with Dr. Roger Pickavance.
http://scholar.google.ca/citations?user=pMEc-oMAAAAJ
A
brief summary of current and past research:
|
Evolutionary interaction
between sexual and asexual Daphnia pulex Daphnia pulex
occurs as two forms, cyclical parthenogens with sexual reproduction and
obligate parthenogens with no sexual reproduction. Both forms co-occur in
southern Ontario but are rarely found in the same pond. We have been
determining sex allocation variation in the cyclical parthenogens and the
avoidance of the cost of males in the obligate parthenogens. Some obligate parthenogen clones can produce males that can mate with
sexual females from the cyclical parthenonogens and
generate new obligately parthenogenetic
clones. Current experiments are using microsatellite genetic markers to
examine competition among males for mating with sexual females and determine
the evolutionary dynamics of the formation of new obligately
parthenogenetic clones. |
|
Ecology and genetics
of Mytilus spp. in Newfoundland Two species of mussel (Mytilus
edulis, M. trossulus)
coexist along the coast of the island of Newfoundland. Limited hybridization
occurs between the species which may be due to incompatibility between
nuclear and cytoplasmic genes. In addition, we are
investigating the role gamete choice may play in limiting hybridization. For additional information see:
http://www.mun.ca/biology/dinnes/Mussels2/Mussels.html http://www.mun.ca/biology/dinnes/Incomplete_Reproductive_Isolation_in_the_Blue_Mussel.htm |
|
Genetic structure of
moss campion (Silene
acaulis) Silene
acaulis occurs as disjunct
populations on the island of Newfoundland. Genetic differentiation appears to
be high and the pattern is not related to geographic distance separating the
populations. Genetic differences between populations on the east and
west side of the island may be the result of different colonization patterns
following post glaciation combined with random
drift and limited gene flow. |
|
Reproductive ecology
of Australian Grevillea spp. Members of the family Proteaceae
have extremely low mature fruit : flower (FR : FL)
ratios compared with other temperate, hermaphroditic, woody perennials. The
role of compatibility in regulating FR : FL ratios
was tested in five closely related species of Grevillea
(Proteaceae). The correlation between self
incompatibility and FR : FL ratios was
non-significant, indicating that compatibility has a minimal effect on fruit
set in the Grevillea species studied. Low FR : FL ratios may result from a combination of pollen
limitation, and high levels of flower and fruit predation. |
|
Reproductive ecology
of Menyanthes trifoliata
in Newfoundland |
|
Genetic structure and
mating behaviour in some Newfoundland spiders
Three species of native ground running spiders were
compared with an introduced orb weaving species for genetic differentiation
over short and long geographic distances. All species showed little evidence
for genetic differentiation. It is unlikely that gene flow is high in these
species and it is possible that large population size reduces the effect of
genetic drift on population differentiation. Studies are also examining the
mating behaviour in the orb weaver Araneus
diadematus to determine if multiple mating
occurs. Preliminary data suggest that if multiple mating occurs it is at a
low level. |
Photo Credits: Daphnia pulex (D. Innes); Mussel
shell (D. Innes); Moss Campion(L. Hermanutz); Grevillea (Plant Systematics);
Menyanthes (T. Schoepke);
Spider (Spider Web Page) |
Teaching
Biology
2900 Principles of Evolution and Systematics
Biology
4250 Evolutionary Genetics
Biology
7290 Evolutionary Ecology
Students
Publications:
On Daphnia:
Innes, D. J. and M. Ginn (2014) A population of sexual Daphnia pulex resists invasion by asexual
clones. Proc. R. Soc. B. 2014 281. http://rspb.royalsocietypublishing.org/content/281/1788/20140564.abstract
Xu, S., Innes, D. J.,
Lynch, M., Cristescu, M. E. (2013) The
role of hybridization in the origin and spread of asexuality in Daphnia. Molecular Ecology 22: 4549 - 4561. http://onlinelibrary.wiley.com/doi/10.1111/mec.12407/abstract
Crease T.J., Floyd R., Cristescu M.E., Innes D.J. (2011) Evolutionary factors
affecting Lactate dehydrogenase A and B variation in
the Daphnia pulex species complex. BMC
Evolutionary Biology 11:212 http://www.biomedcentral.com/1471-2148/11/212
Cristescu M.E., Innes D.J., Stillman J.H., Crease T.J. (2008) D- and L-lactate dehydrogenases during invertebrate evolution. BMC
Evolutionary Biology, 8:
268. http://www.biomedcentral.com/1471-2148/8/268
Fitzsimmons, J. M. and
Innes, D. J. (2006) Inter-genotype variation in reproductive response to
crowding among Daphnia pulex. Hydrobiologia 568: 187-205 (download
PDF file)
Tagg, N., Doncaster, C.P. and Innes, D. J. (2005) Outcomes of
reciprocal invasions between genetically diverse and genetically uniform
populations of Daphnia obtusa (Kurz). Oecologia 143: 527-536(download PDF file)
Fitzsimmons, J. M. and Innes, D. J.
(2005) No evidence of Wolbachia among Great Lakes
area populations of Daphnia pulex (Crustacea: Cladocera). Journal of Plankton Research 27: 121-124 (download PDF file)
Tagg, N., Doncaster, C. P. and Innes, D. J. (2005) Resource
competition between genetically varied and genetically uniform populations of Daphnia
pulex (Leydig): does
sexual reproduction confer a short-term ecological advantage? Biol. J. Linn. Soc. 85: 111-123 (download PDF file)
Winsor, G. L. and Innes, D. J. (2002)
Sexual reproduction in Daphnia pulex (Crustacea: Cladocera):
Observations on male mating behaviour and avoidance of inbreeding. Freshwater Biology 47: 441-450(download PDF file)
Innes, D. J. and Singleton, D. R.
(2000) Variation in allocation to sexual and asexual reproduction among clones
of cyclically parthenogenetic Daphnia pulex (Crustacea: Cladocera). Biol. J. Linn. Soc. 71(4): 771-787
(download PDF
file)
Innes, D. J., Fox, C. J. and Winsor,
G. L. (2000) Avoiding the cost of males in obligately
asexual Daphnia pulex (Leydig).
Proc. Roy. Soc. B 267:
991-997 (download PDF
file)
Innes, D. J. (1997)
Sexual reproduction of Daphnia pulex in a temporary habitat. Oecologia 111: 53-60. (download PDF file)
Innes, D. J. and Singleton, D.
R. (1994) Variation in reproduction and sex allocation among clones
of Daphnia pulex, In Genetics and Evolution of Aquatic Organisms (ed. A.
R. Beaumont), pp. 335-342. London: Chapman and Hall.
Innes, D. J. and Dunbrack, R. L. (1993) Sex allocation variation in Daphnia
pulex.
J. evol. Biol. 6: 559-575.
Innes, D. J. (1991)
Geographic patterns of genetic differentiation among sexual populations of Daphnia
pulex.
Can. J. Zool. 69: 995-1003.
Innes, D. J. (1989) Genetics of Daphnia obtusa:
Genetic load and linkage analysis in a cyclical parthenogen.
Journal of Heredity 80: 6-10.
Innes, D. J. and P.
D. N. Hebert.
(1988) The origin and genetic basis of obligate
parthenogenesis in Daphnia pulex.
Evolution 42: 1024-1035.
Innes, D. J., S. S.
Schwartz and P. D. N. Hebert 1986. Genotypic diversity and
variation in mode of reproduction among populations in the Daphnia pulex group. Heredity 57: 345-355.
Schwartz, S. S., D. J. Innes and P. D.
N. Hebert (1985) Morphological separation of Daphnia pulex and Daphnia obtusa in North America.
Limnology Oceanography 30: 189-197.
On Bivalves:
Lowen, J.B., Innes, D. J.,
Thompson, R. J. (2013) Predator-induced defenses differ between sympatric Mytilus edulis and M. trossulus. Marine Ecology Progress Series,
475:135-143. (download PDF file)
Liu G.X., Stapleton E., Innes D.J.,
Thompson, R.J. (2011) Aggregational behavior of the
blue mussels Mytilus edulis and Mytilus trossulus: a
potential pre-zygotic reproductive isolation mechanism. Marine Ecology 32: 480-487. http://onlinelibrary.wiley.com/doi/10.1111/j.1439-0485.2011.00446.x/abstract
Liu G.X., Innes D.J.,
Thompson R.J. (2011) Quantitative analysis of sperm plane circular movement in
the blue mussels Mytilus edulis, M. trossulus
and their hybrids.
Journal of Experimental Zoology PART A-
Ecological Genetics and Physiology, 315A (5): 280-290. http://onlinelibrary.wiley.com/doi/10.1002/jez.674/abstract
Miranda M.B.B., Innes
D.J., Thompson R.J. (2010) Incomplete reproductive
isolation in the blue mussel (Mytilus edulis and M. trossulus) hybrid zone in the Northwest Atlantic: Role
of gamete interactions and larval viability. Biological Bulletin, 218: 266-281. http://www.biolbull.org/content/218/3/266.full?sid=b06a9774-8172-42fd-a4bd-b3c2e795d4d8
Toro, J. E., R. J. Thompson and D. J. Innes (2006) Fertilization success and early survival in pure and hybrid larvae of Mytilus edulis
(Linnaeus, 1758) and M. trossulus (Gould, 1850) from laboratory crosses. Aquaculture Research 37: 1703-1708
Toro, J. E., D. J. Innes and R. J. Thompson
(2004) Genetic variation among life-history stages of mussels in a Mytilus edulis - M. trossulus hybrid zone. Marine Biology 145: 713-725. (download
PDF file)
Toro, J. E., R. J. Thompson and D. J.
Innes (2002) Reproductive isolation between coexisting mussel species (Mytilus edulis, M. trossulus) in Newfoundland. Marine Biology 141: 897 - 909. (download
PDF file)
Comesana, A. S., Toro, J. E.,
Innes, D. J. and Thompson, R. J. (1999) A molecular approach to the ecology of
a mussel (Mytilus edulis
- M. trossulus) hybrid zone on the east coast of
Newfoundland, Canada. Marine Biology
133: 213-221. (download
PDF file)
Innes, D. J. and
Bates, J. A. (1999) Morphological variation of Mytilus
edulis and Mytilus
trossulus in eastern Newfoundland. Marine Biology 133: 691-699. (download
PDF file)
Jones, R., Bates, J. A., Innes, D. J.
and Thompson, R. J. (1996) A quantitative genetic analysis of growth in larval scallops (Placopecten
magellanicus). Marine Biology 124: 417-424.
Bates, J. A. and Innes,
D. J. (1995) Genetic variation among populations of Mytilus
spp. in eastern Newfoundland. Marine
Biology 124: 417-424.
Muncaster, B. W., D. J. Innes,
P. D. N. Hebert and G. D. Haffner (1989) Patterns of
organic contaminant accumulation by freshwater mussels in the St. Clair River,
Ontario. J. Great Lakes. Res. 15: 645-653.
Koehn, R. K., J. G. Hall, D. J. Innes
and A. J. Zera (1984) Genetic differentiation of Mytilus edulis in
eastern North America. Mar. Biol. 79: 117-126.
Innes, D. J. and L. E. Haley (1977)a. Inheritance of a shell color polymorphism in the mussel.
J. of Heredity 68: 203-204. http://jhered.oxfordjournals.org/content/68/3/203.full.pdf+html
Innes, D. J. and L. E. Haley (1977)b. Genetic aspects of larval growth under reduced salinity
in Mytilus edulis. Biol.
Bull. 153: 312-321. http://www.biolbull.org/content/153/2/312.full.pdf+html?sid=b06a9774-8172-42fd-a4bd-b3c2e795d4d8
On plant mating systems and population genetics:
Thompson, F. L., Hermanutz,
L. A. and Innes, D. J. (1999) The reproductive
ecology of island populations of distylous Menyanthes trifoliata
(menyanthaceae). Can.
J. Bot. 76: 818-828. (download PDF file)
Hermanutz, L. A., Innes, D.
J., Denham, A. and Whelan, R. (1998) Very low fruit: flower ratios in Grevillea (Proteaceae) are
independent of breeding system. Australian
J. of Botany 46: 465-478. (download
PDF file)
Rajora, O. P., Deverno, L., Mosseler, A. and
Innes, D. J. (1998) Genetic diversity and population structure of disjunct Newfoundland and central Ontario populations of
eastern white pine (Pinus strobus). Can. J.
Bot. 76: 500-508. (download PDF file)
Hermanutz,
L. A. and Innes, D. J. (1994) Gender variation in Silene
acaulis (Caryophyllaceae). Plant Sytematics and Evolution 191:
69-81.
Mosseler,
A., D. J. Innes and B. A. Roberts. (1991) Lack of allozymic
variation in disjunct Newfoundland populations of red
pine (Pinus resinosa).
Can. J. For. Res. 21: 525-528.
Innes, D. J. (1990) Microgeographic genetic structure in the haploid and
diploid stages of the moss Polytrichum juniperinum Hedw. Heredity
64: 331-340.
Innes, D. J. and G.
S. Ringius (1990) Mating system and genetic structure
of two populations of white spruce (Picea glauca) in eastern Newfoundland. Can. J. Bot. 68:
1661-1666.
Ringius,
G. S. and D. J. Innes. (1990) Large disparity in
recombination fractions reported in Picea mariana for the Aat-1/Pgi-2 linkage group. Can. J. Bot.
68: 518-520.
Hermanutz,
L. A., D. J. Innes and I. M. Weis. (1989) Clonal
structure of dwarf birch, Betula glandulosa, at its northern limit. Amer. J. Bot.
76: 755-761.
Innes, D. J. and L.
A. Hermanutz (1988) Mating system and genetic
structure in a disjunct population of the seaside
goldenrod, Solidago sempervirens. Heredity 61:
447-454.
Innes, D. J. (1988)
Genetic differentiation in the intertidal zone for the alga Enteromorpha linza (Ulvales,
Chlorophyta). Mar. Bio. 97: 9-16.
Innes, D. J. (1987) The
genetic structure of asexually reproducing Enteromorph linza (Ulvales,
Chlorophyta) in Long Island Sound. Marine Biology 94:
459-467.
Innes, D. J. (1984)
Genetic differentiation among populations of marine algae. Helgolander
Meersuntersuchungen 38: 401-417.
Innes, D. J. and C. Yarish (1984) Genetic evidence for the occurrence of
asexual reproduction in populations of Enteromorpha linza (L.)J. Ag. (Chlorophyta,
Ulvales) from Long Island sound. Phycologia 23: 311-320.
Other publications:
Zera, A. J., D. J. Innes
and M. E. Saks (1983) Genetic and environmental determinants of wing
polymorphisms in the waterstrider Limnoporus canaliculatus. Evolution 37: 513-522.
Smith, G. R., J. G. Hall, R. K. Koehn
and D. J. Innes (1983) Taxonomic relationships of the Zuni mountain
sucker, Catostomus discobolus yarrowi. Copeia 1983: 37-48.